Hoʻohana mākou i nā kuki e hoʻonui i kou ʻike.Ma ka hoʻomau ʻana i ka nānā ʻana i kēia pūnaewele, ʻae ʻoe i kā mākou hoʻohana ʻana i nā kuki.ʻIke hou aku.
Pono e hoʻomaʻamaʻa ʻia nā hōʻike noiʻi biomedical o nā pūnana mammalian i ʻoi aku ka maʻamau a me ka kikoʻī, a i ʻoi aku ka maikaʻi o ka mālama ʻana a me ke ana ʻana i nā kūlana kaiapuni o ka moʻomeheu cell.ʻO kēia ka mea e ʻoi aku ka pololei o ka hoʻohālikelike ʻana i ka physiology kanaka a kōkua i ka reproducibility o ka noiʻi.
Ua kālailai kekahi hui o KAUST ʻepekema a me nā hoa hana ma Saudi Arabia a me ʻAmelika Hui Pū ʻIa i 810 mau pepa i koho ʻia ma nā laina kelepona mammalian.ʻOi aku ka liʻiliʻi ma mua o 700 o lākou i komo i ka 1,749 mau hoʻokolohua moʻomeheu kelepona hoʻokahi, me ka ʻikepili kūpono e pili ana i nā kūlana kaiapuni o ke ʻano moʻomeheu cell.Hōʻike ka hōʻike ʻana o ka hui he nui nā hana e pono ai e hoʻomaikaʻi i ka pili a me ka hana hou ʻana o ia mau haʻawina.
E hoʻoulu i nā pūnaewele i loko o kahi incubator i hoʻomaluʻia e like me nā protocol maʻamau.Akā, e ulu ana nā pūnaewele a "hanu" i ka manawa, e hoʻololi i ke kinoea me ke kaiapuni a puni.E pili ana kēia i ka nohona kūloko kahi e ulu ai lākou, a hiki ke hoʻololi i ka ʻakika o ka moʻomeheu, oxygen dissolved, a me nā ʻāpana kalapona kalapona.Hoʻololi kēia mau hoʻololi i ka hana kelepona a hiki ke hoʻololi i ke ʻano o ke kino mai ke ʻano o ke kino kanaka ola.
"Ke koʻikoʻi nei kā mākou noiʻi i ka nui o ka haʻalele ʻana o ka poʻe ʻepekema i ka nānā ʻana a me ka mālama ʻana i ke kaiapuni kelepona, a i ka nui o nā hōʻike e hiki ai iā lākou ke hōʻea i nā hopena ʻepekema ma nā ʻano kikoʻī," wahi a Klein.
No ka laʻana, ua ʻike nā mea noiʻi ma kahi o ka hapalua o nā pepa analytical ʻaʻole i hōʻike i ka mahana a me nā hoʻonohonoho carbon dioxide o kā lākou moʻomeheu cell.Ma lalo o 10% i hōʻike i ka ʻike o ka oxygen ea i loko o ka incubator, a ma lalo o 0.01% i hōʻike i ka acidity o ka medium.ʻAʻohe pepa i hōʻike ʻia e pili ana i ka oxygen dissolved a i ʻole carbon dioxide ma ka pāpā.
Pīhoihoi nui mākou i ka nānā ʻole ʻana o ka poʻe noiʻi i nā mea kūlohelohe e mālama i nā pae pili physiologically i ka wā o ke kaʻina holoʻokoʻa o ka moʻomeheu cell, e like me ka acidity moʻomeheu, ʻoiai ua ʻike ʻia he mea nui kēia no ka hana cell.”
Ke alakaʻi ʻia nei ka hui e Carlos Duarte, he kanaka ʻepekema moana ma KAUST, a ʻo Mo Li, he mea ola ola kino, me ka hui pū ʻana me Juan Carlos Izpisua Belmonte, he mea hoʻomohala hoʻomohala ma ka Salk Institute.I kēia manawa he polopeka kipa ʻo ia ma KAUST a paipai i nā ʻepekema biomedical e hoʻomohala i nā hōʻike maʻamau A me nā kaʻina hana hoʻomalu a me ke ana ʻana, ma waho aʻe o ka hoʻohana ʻana i nā mea hana kūikawā e hoʻomalu i ka nohona moʻomeheu o nā ʻano cell like ʻole.Pono nā puke pai ʻepekema e hoʻokumu i nā kūlana hōʻike a koi aku i ka nānā pono ʻana a me ka mālama ʻana i ka acidity media, dissolved oxygen a me carbon dioxide.
"ʻOi aku ka maikaʻi o ka hōʻike ʻana, ana, a me ka hoʻomalu ʻana i nā kūlana kaiapuni o ka moʻomeheu cell e hoʻomaikaʻi i ka hiki o nā ʻepekema ke hana hou a hana hou i nā hopena hoʻokolohua," wahi a Alsolami."Hiki i ka nānā ʻana i nā mea hou a hoʻonui i ka pili o ka noiʻi preclinical i ke kino kanaka."
"ʻO ka moʻomeheu mammalian cell ke kumu no ka hana ʻana i nā lāʻau lapaʻau maʻi a me nā biotechnologies ʻē aʻe," wehewehe ʻo Shannon Klein ka mea ʻepekema moana."Ma mua o ka hoʻāʻo ʻana i nā holoholona a me nā kānaka, hoʻohana ʻia lākou e aʻo i ka biology cell basic, replicate mechanical mechanisms, and study the toxic of new drug compound."
Klein, SG, etc. (2021) Pono nā hana maikaʻi loa i ka mālama ʻole ʻana i ke kaiapuni i ka moʻomeheu cell mammalian.ʻEnekinia Biomedical Natural.doi.org/10.1038/s41551-021-00775-0.
Tags: B cell, cell, cell culture, incubator, mammalian cell, manufacturing, oxygen, pH, physiology, preclinical, noiʻi, T cell
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Hāʻawi ʻo News-Medical.Net i kēia lawelawe ʻike olakino e like me kēia mau ʻōlelo a me nā kūlana.E ʻoluʻolu e hoʻomaopopo i ka ʻike olakino ma kēia pūnaewele i manaʻo ʻia e kākoʻo ma mua o ka hoʻololi ʻana i ka pilina ma waena o nā maʻi a me nā kauka/kauka a me nā ʻōlelo aʻo a lākou e hāʻawi ai.
Ka manawa hoʻouna: Sep-07-2021
